High molecular mass intracellular proteases

High molecular mass intracellular proteases.

Many of the well-characterized proteolytic enzymes, and particularly those for which X-ray structures are now available, are small monomeric enzymes often having molecular masses in the range of 20-30 kDa. Many of them are extracellular enzymes which are easy to assay and to purify. With a growing awareness of the importance of intracellular protein turnover and mechanisms of intracellular prot...

Extracting Intracellular Proteases from Bacteria'

VADEHRA, D. V. (Michigan State University, East Lansing), D. L. WALLACE, AND L. G. HARMON. Comparison of methods of extracting intracellular proteases from bacteria. Appl. Microbiol. 13:1010-1013. 1965.-Five commonly used methods of disintegrating bacterial cells were compared by use of Bacillus subtilis, Pseudomonas putrefaciens, and Streptococcus durans as the test organisms. These methods we...

Partial Purification of Mucor pusillus Intracellular Proteases.

The intracellular protease extracted from the freeze-dried mycelia obtained after the growth of Mucor pusillus at 30 degrees C in corn steep liquor medium was chromatographed on DEAE-A50. Some characteristics of the protease fractions obtained after ion-exchange chromatography were determined and compared with those of the extracellular proteases reported previously. The mycelia were found to c...

Proteases and Anti-proteases: Molecular Mechanisms and Novel Therapeutic Targets

a,-Antitrypsin functions as a 'mousetrap ' to inhibit its target proteinase, neutrophil elastase. The common severe Z deficiency variant ( G ~ u ~ ~ ' + Lys) destabilizes the mousetrap to allow a sequential protein-protein interaction between the reactive-centre loop of one molecule and psheet A of another. These loop-sheet polymers accumulate within hepatocytes to form inclusion bodies that ar...

Modeling Spatial Distribution of Intracellular Organelles by Molecular Motors